A Two-Photon Ratiometric Fluorescent Probe for Imaging Carboxylesterase 2 in Living Cells and Tissues.

作者: Jin Q, Feng L, Wang DD, Dai ZR, Wang P, Zou LW, Liu ZH, Wang JY, Yu Y, Ge GB, Cui JN, Yang L
成果类型: 期刊论文
数据来源: ESI SCIE EI
发表日期: 2015
年卷期页: 2015,7,(51),28474-81
期刊名: 《ACS applied materials & interfaces》 影响因子和分区 年份    影响因子    区号
2016    7.504        3区 2015    7.145        3区 2014    6.723        3区 2013    5.008        3区 2012    4.540        3区
英文关键词: bioimaging;carboxylesterase 2;ratiometric fluorescent probe;specific sensing;two-photon
英文摘要: In this study, a two-photon ratiometric fluorescent probe NCEN has been designed and developed for highly selective and sensitive sensing of human carboxylesterase 2 (hCE2) based on the catalytic properties and substrate preference of hCE2. Upon addition of hCE2, the probe could be readily hydrolyzed to release 4-amino-1,8-naphthalimide (NAH), which brings remarkable red-shift in fluorescence (90 nm) spectrum. The newly developed probe exhibits good specificity, ultrahigh sensitivity, and has been successfully applied to determine the real activities of hCE2 in complex biological samples such as cell and tissue preparations. NCEN has also been used for two-photon imaging of intracellular hCE2 in living cells as well as in deep-tissues for the first time, and the results showed that the probe exhibited high ratiometric imaging resolution and deep-tissue imaging depth. All these findings suggested that this probe holds great promise for applications in bioimaging of endogenous hCE2 in living cells and in exploring the biological functions of hCE2 in complex biological systems.
语言: United States
出版类型: Journal Article
作者地址: Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China. [1]
State Key Laboratory of Fine Chemicals, Dalian University of Technology , Dalian 116012, China. [2]
Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China. [3]
Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China. [4]
Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China. [5]
Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China. [6]
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, China. [7]
Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China. [8]
Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China. [9]
Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China. [10]
State Key Laboratory of Fine Chemicals, Dalian University of Technology , Dalian 116012, China. [11]
Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China. [12]
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