Simultaneous qualitation and quantification of four phytoecdysones in Radix Achyranthis Bidentatae by high-performance liquid chromatography with diode array detection
|作者：||Li J, Li HJ, Li P, Qi H|
|期刊名：||《BIOMEDICAL CHROMATOGRAPHY》 影响因子和分区||
2017 1.688 4区 2016 1.613 4区 2015 1.729 4区 2014 1.723 4区 2013 1.945 4区
|英文关键词：||HPLC-DAD; phytoecdysones; Radix Achyranthis Bidentatae; quantification|
|英文摘要：||A high-performance liquid chromatographic method with diode array detection was developed and validated to simultaneously identify and quantify four phytoecdysones, i.e., polypodine B (1), ecdysterone (2), 25-R inokosterone (3) and 25-S inokosterone (4), in Radix Achyranthis Bidentatae. The analysis was performed using a C-18 column with 6% tetrahydrofuran aqueous and acetonitrile isocratic elution, and the detection was carried out by DAD at 242 nm. The identities of the analytes were determined by comparing the retention time and UV spectrum with those of reference compounds. The validation of the method included linearity, sensitivity, recovery and stability. All calibration curves of the four phytoecdysones showed good linear regression (r(2) >= 0.9993). The limit of detection (S/N = 3) and limit of quantification (S/N = 10) were less than 7.5 and 12.3 ng, respectively. The method provided desirable repeatability with overall intra- and inter-day variations of less than 4.67%. The obtained recoveries varied between 95.1 and 104.4% while the relative standard deviations were below 4.85% (n = 3). The analysis results indicated that the contents of the investigated phytoecdysones in Radix Achyranthis Bidentatae from different locations were highly variant, and the genuine crude drug indigenous to Henan province did not possess the highest concentration of phytoecdysones. Copyright (C) 2007 John Wiley & Sons, Ltd.|
|主题：||Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical; Pharmacology & Pharmacy;Biochemistry & Molecular Biology; Chemistry; Pharmacology & Pharmacy|
|作者地址：||China Pharmaceut Univ, Key Lab Modern Chinese Med, Minist Educ, Nanjing 210009, Peoples R China; China Pharmaceut Univ, Dept Pharmacognosy, Nanjing 210009, Peoples R China|
|出版社：||JOHN WILEY & SONS LTD|
|出版地址：||THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND|
|参考文献：||[？？？？ Bai Yan], 2005, [？в？？, Chinese Traditional and Herbal Drugs], V36, P1391
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