Simultaneous qualitation and quantification of four phytoecdysones in Radix Achyranthis Bidentatae by high-performance liquid chromatography with diode array detection

作者: Li J, Li HJ, Li P, Qi H
学科名称: 医学
成果类型: 期刊论文
数据来源: ESI SCIE
发表日期: 2007
年卷期页: 2007,21,(8),823-828
期刊名: 《BIOMEDICAL CHROMATOGRAPHY》 影响因子和分区 年份    影响因子    区号
2017    1.688        4区 2016    1.613        4区 2015    1.729        4区 2014    1.723        4区 2013    1.945        4区
缩写刊名: BIOMED CHROMATOGR
英文关键词: HPLC-DAD; phytoecdysones; Radix Achyranthis Bidentatae; quantification
英文摘要: A high-performance liquid chromatographic method with diode array detection was developed and validated to simultaneously identify and quantify four phytoecdysones, i.e., polypodine B (1), ecdysterone (2), 25-R inokosterone (3) and 25-S inokosterone (4), in Radix Achyranthis Bidentatae. The analysis was performed using a C-18 column with 6% tetrahydrofuran aqueous and acetonitrile isocratic elution, and the detection was carried out by DAD at 242 nm. The identities of the analytes were determined by comparing the retention time and UV spectrum with those of reference compounds. The validation of the method included linearity, sensitivity, recovery and stability. All calibration curves of the four phytoecdysones showed good linear regression (r(2) >= 0.9993). The limit of detection (S/N = 3) and limit of quantification (S/N = 10) were less than 7.5 and 12.3 ng, respectively. The method provided desirable repeatability with overall intra- and inter-day variations of less than 4.67%. The obtained recoveries varied between 95.1 and 104.4% while the relative standard deviations were below 4.85% (n = 3). The analysis results indicated that the contents of the investigated phytoecdysones in Radix Achyranthis Bidentatae from different locations were highly variant, and the genuine crude drug indigenous to Henan province did not possess the highest concentration of phytoecdysones. Copyright (C) 2007 John Wiley & Sons, Ltd.
语言: English
出版类型: Article
主题: Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical; Pharmacology & Pharmacy;Biochemistry & Molecular Biology; Chemistry; Pharmacology & Pharmacy
作者邮箱: cpuli@163.com  
作者地址: China Pharmaceut Univ, Key Lab Modern Chinese Med, Minist Educ, Nanjing 210009, Peoples R China; China Pharmaceut Univ, Dept Pharmacognosy, Nanjing 210009, Peoples R China
出版社: JOHN WILEY & SONS LTD
出版地址: THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND
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