Metabolomics Study on the Effects of Jieduquyuziyin Prescription on Systemic Lupus Erythematosus Mice by LC-Q-TOF/MS

作者: Hu JB, Jiang FS, Gu HC, Ding ZS, Yao L, Fan YS, Ding XH
学科名称: 医学
成果类型: 期刊论文
数据来源: ESI SCIE
发表日期: 2013
年卷期页: 2013,76,(13-14),791-800
期刊名: 《CHROMATOGRAPHIA》 影响因子和分区 年份    影响因子    区号
2016    1.402        4区 2015    1.332        4区 2014    1.411        4区 2013    1.437        4区 2012    1.275        4区
英文关键词: HPLC-Q-TOF/MS; Metabolomics; PCA; PLS-DA; Systemic lupus erythematosus; Jieduquyuziyin prescription
英文摘要: A metabolomics approach was applied to study the metabolites characters of systemic lupus erythematosus (SLE) and therapeutical effects of jieduquyuziyin prescription (JP). High performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF/MS) in positive mode was employed to analyze urine specimens of the SLE model mice (MRL/lpr) group, prednisone acetate (PA)-treated SLE mice group, JP-treated SLE mice group, and control group (C57BL/6 J). Software of Mass Hunter and Mass Profiler Professional (MPP) were used to process the data. Multivariate statistical analysis of principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were applied to recognize the data pattern. PCA result showed that there were obvious disorders of amino acid metabolism in SLE model. Ten compounds have significant contributions to classification were identified as differential metabolites, such as glycine, pantetheine, and phenylpyruvic acid. Metabolism of phenylalanine tended to recover to the normal state after treatment with PA. The changes of glycine, phenylalanine, and tryptophan in SLE mice showed some alleviation in JP-treated SLE group. Thus, the JP has a good therapeutic effect on the SLE model mice by regulating the dysfunctions of amino acid metabolism. These results may be helpful to further understand the pathological mechanisms of SLE from the whole metabolism.
语言: English
出版类型: Article
主题: Biochemical Research Methods; Chemistry, Analytical;Biochemistry & Molecular Biology; Chemistry
作者地址: [Hu, Jin-bo; Jiang, Fu-sheng; Gu, Heng-cun; Ding, Zhi-shan; Yao, Li; Fan, Yong-sheng; Ding, Xing-hong] Zhejiang Chinese Med Univ, Hangzhou 310053, Zhejiang, Peoples R China
基金支持数量: National Natural Science Foundation of China [81001515]
Research Fund for the Doctoral Program of Higher Education of China [2009332 2120001]
基金: This study has been supported by the National Natural Science Foundation of China (NO. 81001515), Research Fund for the Doctoral Program of Higher Education of China (NO. 2009332 2120001)
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