Global detection and identification of components from crude and processed traditional Chinese medicine by liquid chromatography connected with hybrid ion trap and time-of-flight-mass spectrometry

作者: Cao G, Zhang CR, Zhang Y, Cong XD, Cai H, Cai BC, Li XM, Yao JT
学科名称: 医学
成果类型: 期刊论文
数据来源: ESI SCIE EI
发表日期: 2011
年卷期页: 2011,34,(15),1845-1852
期刊名: 《JOURNAL OF SEPARATION SCIENCE》 影响因子和分区 年份    影响因子    区号
2017    2.415        3区 2016    2.557        3区 2015    2.741        3区 2014    2.737        3区 2013    2.591        3区
缩写刊名: J SEP SCI
英文关键词: Crude and processed TCM; Fructus corni; Liquid chromatography ion trap-time of-flight-mass spectrometry; Quality control
英文摘要: We herein present a chemical profiling method to efficiently process the information acquired by ultra fast liquid chromatography (UFLC)-electrospray ionization source in combination with hybrid ion trap and high-resolution time-of-flight mass spectrometry (UFLC-(ESI)-IT-TOF/MS), facilitating the structural determination of serial components contained in crude or processed traditional Chinese medicine (TCM). Under the optimized UFLC and IT-TOF-MSn conditions, over 39 compounds were separated and detected in crude or processed Fructus corni within 25 min. The components were identified by comparing the mass spectra and retention time with reference compounds, or tentatively assigned by elucidating low-energy collision-induced dissociation (CID) fragment ions and matching empirical molecular formula with that of the published compounds. Several factors in the processing procedure were examined. The experimental results demonstrate that the chemical reactions that occurred in the processing procedure can be used to elucidate the processed mechanism of F. corni, which is regularly affected by the processing conditions. This study provides a novel approach and methodology to identify the complicated components from various complex mixtures such as crude TCM, processed TCM, and biological samples. It can be used as a valid analytical method for further understanding the processing mechanism of TCM, along with the intrinsic quality control of TCM and its processed product.
语言: English
出版类型: Article
主题: Chemistry, Analytical;Chemistry
作者邮箱: bccai@hotmail.com  
作者地址: [Cai, Hao; Cai, Baochang] Nanjing Univ Chinese Med, Engn Res Ctr, Minist Educ Standardizat Chinese Med Proc, Nanjing 210029, Peoples R China
[Cao, Gang; Zhang, Chengrong; Zhang, Yun; Cong, Xiaodong; Cai, Baochang; Li, Xiaomeng] Zhejiang Chinese Med Univ, Res Ctr TCM Proc Technol, Hangzhou, Zhejiang, Peoples R China
[Yao, Jinting] Shimadzu Int Trading Shanghai, Analyt Applicat Ctr, Analyt Instruments Dept, Shanghai, Peoples R China
基金支持数量: National Science Foundation of China [30873438]
Science and Technology of Chinese Medicine Foundation of Zhejiang Province [2009CB008]
基金: This work was financially supported by the National Science Foundation of China (No. 30873438) and Science and Technology of Chinese Medicine Foundation of Zhejiang Province (No. 2009CB008).
出版社: WILEY-V C H VERLAG GMBH
出版地址: BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY
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